Learn vocabulary, terms, and more with flashcards, games, and other study tools. Download free adobe acrobat reader dc software for your windows, mac os and android devices to view, print, and comment on pdf documents. The principle of the biuret assay is similar to that of the lowry, however it involves a single incubation of 20 min. For product supporttroubleshooting, please email us your inquiry at. Download as docx, pdf, txt or read online from scribd. Total protein biuret reagent ec rep spectrum diagnostics. There are very few interfering agents ammonium salts being one such agent, and layne 1957 reported fewer deviations than with the lowry or ultraviolet absorption methods. Biuret is a chemical compound with the chemical formula h2nco2nh. Although there is a small amount of urea in feedgrade biuret, it is tightly imbedded in the biuret crystals.
The estimation of protein by the biuret and greenberg methods. Links to pubmed are also available for selected references. The hartreelowry method was developed and implemented for quantification of. The test and reagent do not actually contain biuret. The biuret test is a chemical test for proteins and polypeptides. Assay principle in alkaline medium the copper reacts with the peptide bonds of proteins to form the characteristic pink to purple biuret complex. Full text full text is available as a scanned copy of the original print version. Scribd is the worlds largest social reading and publishing site. It is based on the biuret reagent, a blue solution that turns violet upon contact with proteins, or any substance with peptide bonds. However, it is interesting to see that a crude mixture, the lowry technique can be.
196 70 291 1373 171 482 1256 1229 957 809 52 762 855 70 938 1080 874 1456 671 893 434 305 1012 1163 784 477 1333 924 2 759 118 710